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macs cell separation column ls miltenyi biotec  (Miltenyi Biotec)


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    Miltenyi Biotec macs cell separation column ls miltenyi biotec
    Macs Cell Separation Column Ls Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 8894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macs+separation+column+ls/LS+Columns/pm42248140-238-219-224
    Average 99 stars, based on 8894 article reviews
    macs cell separation column ls miltenyi biotec - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: B-lineage commitment is dependent on a reversible epigenetic switch.
    Article Snippet: .. Cells were incubated with 25 μL of CD117microbeads (Miltenyi Biotec 130-091-224) per 100 × 106 cells with rotation for 20 min at 4°C, followed by enrichment of c-Kit-positive cells by using MACS separation column LS (Miltenyi Biotec 130-042-401). .. Sorting of BM progenitors for single-cell multiome sequencing For isolation of BM progenitors for sc-multiome sequencing, the following antibody cocktails in Tingvall-Gustafsson et al. 12 GENES & DEVELOPMENT PBS/0.1% FCS were used: LSK [live/dead near-IR (Life Technologies L34975), Lin (PECy5), Sca-1 (D7 and PECy7, eBioscience 25-5981-82), and CD117(c-KIT) (2B8 and PE, BioLegend, 105808)], FLT3+ [live/dead near-IR (Life Technologies L34975), Lin (PECy5), and CD135 (Flt3) (A2F10.1 and PE, BD Biosciences 553842)], IL7RA+ [live/dead near-IR (Life Technologies L34975), Lin (PECy5), and CD127(Il7Ra) (AF1234 and PE, BioLegend 135010)], and CD19+ [live/dead near-IR (Life Technologies L34975), Lin (PECy5), CD19 (6D5 and PE, BioLegend 115508), IgD (11-26c.2a and APC, BioLegend 405714), and IgM (II/41 andAPC, eBioscience 17-5790-82)].

    Article Title: High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes
    Article Snippet: Tonsils were obtained from patients undergoing tonsillectomies (HELIOS Klinikum, Erfurt, Germany). .. Isolated cells from tonsils were incubated with CD19 micro beads (Miltenyi, Germany) and loaded in a magnetic field on a MACS separation column LS (Miltenyi). ..

    Article Title: B-lineage commitment is dependent on a reversible epigenetic switch
    Article Snippet: .. Cells were incubated with 25 μL of CD117 microbeads (Miltenyi Biotec 130-091-224) per 100 × 10 6 cells with rotation for 20 min at 4°C, followed by enrichment of c-Kit-positive cells by using MACS separation column LS (Miltenyi Biotec 130-042-401). .. For isolation of BM progenitors for sc-multiome sequencing, the following antibody cocktails in PBS/0.1% FCS were used: LSK [live/dead near-IR (Life Technologies L34975), Lin (PECy5), Sca-1 (D7 and PECy7, eBioscience 25-5981-82), and CD117(c-KIT) (2B8 and PE, BioLegend, 105808)], FLT3 + [live/dead near-IR (Life Technologies L34975), Lin (PECy5), and CD135(Flt3) (A2F10.1 and PE, BD Biosciences 553842)], IL7RA + [live/dead near-IR (Life Technologies L34975), Lin (PECy5), and CD127(Il7Ra) (AF1234 and PE, BioLegend 135010)], and CD19 + [live/dead near-IR (Life Technologies L34975), Lin (PECy5), CD19 (6D5 and PE, BioLegend 115508), IgD (11-26c.2a and APC, BioLegend 405714), and IgM (II/41 and APC, eBioscience 17-5790-82)].

    Magnetic Cell Separation:

    Article Title: B-lineage commitment is dependent on a reversible epigenetic switch.
    Article Snippet: .. Cells were incubated with 25 μL of CD117microbeads (Miltenyi Biotec 130-091-224) per 100 × 106 cells with rotation for 20 min at 4°C, followed by enrichment of c-Kit-positive cells by using MACS separation column LS (Miltenyi Biotec 130-042-401). .. Sorting of BM progenitors for single-cell multiome sequencing For isolation of BM progenitors for sc-multiome sequencing, the following antibody cocktails in Tingvall-Gustafsson et al. 12 GENES & DEVELOPMENT PBS/0.1% FCS were used: LSK [live/dead near-IR (Life Technologies L34975), Lin (PECy5), Sca-1 (D7 and PECy7, eBioscience 25-5981-82), and CD117(c-KIT) (2B8 and PE, BioLegend, 105808)], FLT3+ [live/dead near-IR (Life Technologies L34975), Lin (PECy5), and CD135 (Flt3) (A2F10.1 and PE, BD Biosciences 553842)], IL7RA+ [live/dead near-IR (Life Technologies L34975), Lin (PECy5), and CD127(Il7Ra) (AF1234 and PE, BioLegend 135010)], and CD19+ [live/dead near-IR (Life Technologies L34975), Lin (PECy5), CD19 (6D5 and PE, BioLegend 115508), IgD (11-26c.2a and APC, BioLegend 405714), and IgM (II/41 andAPC, eBioscience 17-5790-82)].

    Article Title: Class IIa HDAC inhibition reduces breast tumors and metastases via anti-tumor macrophages
    Article Snippet: Whole tumor cell suspension was incubated with biotinylated anti-EPCAM antibody (MACS Miltenyi cat. #130-101-859, clone caa7-9G8) for 10 minutes followed by incubation with Anti-Biotin Microbeads (MACS Miltenyi cat. #130-090-485) for 15 minutes at 4°C in the dark. .. Cells were washed in ice cold PBS containing 0.5% BSA and 2mM EDTA (Sigma-Aldrich cat. #E7889) (pH 7.2), and loaded onto a MACS Separation Column LS (Miltenyi cat. #130-042-401) appropriately secured on a MidiMACS Separator magnet (Miltenyi cat. #130-042-302). .. Following negative selection, EPCAM-depleted cells were stained for anti-mouse CD45 Alex Flour ® 488 (BioLegend cat. #103121, clone 30-F11), anti-mouse CD3 BV421 (BioLegend cat. #100227, clone 17A2), anti-mouse CD11b APC (BioLegend cat. #101211, clone M1/70), and anti-mouse CD19 (BioLegend cat. #115507, clone 6D5).

    Article Title: High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes
    Article Snippet: Tonsils were obtained from patients undergoing tonsillectomies (HELIOS Klinikum, Erfurt, Germany). .. Isolated cells from tonsils were incubated with CD19 micro beads (Miltenyi, Germany) and loaded in a magnetic field on a MACS separation column LS (Miltenyi). ..

    Article Title: B-lineage commitment is dependent on a reversible epigenetic switch
    Article Snippet: .. Cells were incubated with 25 μL of CD117 microbeads (Miltenyi Biotec 130-091-224) per 100 × 10 6 cells with rotation for 20 min at 4°C, followed by enrichment of c-Kit-positive cells by using MACS separation column LS (Miltenyi Biotec 130-042-401). .. For isolation of BM progenitors for sc-multiome sequencing, the following antibody cocktails in PBS/0.1% FCS were used: LSK [live/dead near-IR (Life Technologies L34975), Lin (PECy5), Sca-1 (D7 and PECy7, eBioscience 25-5981-82), and CD117(c-KIT) (2B8 and PE, BioLegend, 105808)], FLT3 + [live/dead near-IR (Life Technologies L34975), Lin (PECy5), and CD135(Flt3) (A2F10.1 and PE, BD Biosciences 553842)], IL7RA + [live/dead near-IR (Life Technologies L34975), Lin (PECy5), and CD127(Il7Ra) (AF1234 and PE, BioLegend 135010)], and CD19 + [live/dead near-IR (Life Technologies L34975), Lin (PECy5), CD19 (6D5 and PE, BioLegend 115508), IgD (11-26c.2a and APC, BioLegend 405714), and IgM (II/41 and APC, eBioscience 17-5790-82)].

    Article Title: Method of Microglial DNA-RNA Purification from a Single Brain of an Adult Mouse
    Article Snippet: QIAshredder (REF79654) , Qiagen , 160010887 , Hilden, Germany. .. MACS separation column (LS) , Miltenyi Biotec , 130-042-401 , Auburn, CA, USA. .. Disposable vacuum filter, 0.22 μm (50 mL) , Millipoe Sigma , SCGP00525 , Burlington, MA, USA.

    Isolation:

    Article Title: High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes
    Article Snippet: Tonsils were obtained from patients undergoing tonsillectomies (HELIOS Klinikum, Erfurt, Germany). .. Isolated cells from tonsils were incubated with CD19 micro beads (Miltenyi, Germany) and loaded in a magnetic field on a MACS separation column LS (Miltenyi). ..



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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and <t>MACS-purified</t> glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic <t>of</t> <t>magnetic</t> beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using anti-O4, anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and <t>MACS-purified</t> glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic <t>of</t> <t>magnetic</t> beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using anti-O4, anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and <t>MACS-purified</t> glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic <t>of</t> <t>magnetic</t> beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using anti-O4, anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.
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    A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using anti-O4, anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.

    Journal: bioRxiv

    Article Title: Injury-transduced SerpinOLs modulate neuroinflammation and glial activation in the diseased and non-diseased CNS

    doi: 10.64898/2026.01.29.702669

    Figure Lengend Snippet: A , experimental design. Inflammatory demyelination of experimental autoimmune encephalomyelitis (EAE) was induced in adult mice via MOG immunization (CFA as control) at day 0 (D0), with pertussis administered i.p. on D0 and D2. RNA-seq was performed on whole spinal cords ( B ) and MACS-purified glial populations ( C-F ) at D30-D35. OLs, oligodendrocytes; Astro, astrocytes; MG, microglia. B , volcano plot of differentially expressed genes (DEGs) in spinal cord of MOG/EAE (n=4) vs. CFA mice (n=3) at D35. Top10 DEGs are labeled with gene symbols and FPKM values. C , schematic of magnetic beads-assisted cell sorting (MACS) of spinal cord glia at D30. OL, Astro, and myeloid cells (including MG) were isolated using anti-O4, anti-ACSA2, and anti-CD11b magnetic beads, respectively. D , purity validation of sorted population using representative markers Plp1 (OL), Tmem119 (MG), and Aldh1l1 (Astro). Expression is shown as TPM (transcripts per million). E , volcano plot of DEGs in MOG_OL versus CFA_OL. Serpina3n is highlighted in red. F , TPM expression of Serpina3n , Ctsd , Lgals3 , and Gpnmb in OLs, Astro, and MG from MOG/EAE and CFA mice. Serpina3n is specifically upregulated in OL, while Ctsd , Lgals3 , and Gpnmb are enriched and upregulated in MG.

    Article Snippet: To magnetic separate the glial cells magneteiclly, the LS MACS Column was positioned in the magnetic field of a MACS Separator (Cat# 130-090-976, Miltenyi Biotec) and rinsed with 3 mL of 0.5% BSA/PBS buffer.

    Techniques: Control, RNA Sequencing, Purification, Labeling, Magnetic Beads, FACS, Isolation, Biomarker Discovery, Expressing